CopySwitch-in vivo Optimization of Gene Copy Numbers for Heterologous Gene Expression in Bacillus subtilis.

ABSTRACT: The Gram-positive bacterium Bacillus subtilis has long been used as a host for production and secretion of industrially relevant enzymes like amylases and proteases. It is imperative for optimal efficiency, to balance protein yield and correct folding. While there are numerous ways of doing so on protein or mRNA level, our approach aims for the underlying number of coding sequences. Gene copy numbers are an important tuning valve for the optimization of heterologous gene expression. While some genes are best expressed from many gene copies, for other genes, medium or even single copy numbers are the only way to avoid formation of inclusion bodies, toxic gene dosage effects or achieve desired levels for metabolic engineering. In order to provide a simple and robust method to address

SUBMITTER: Nadler F 

PROVIDER: S-EPMC6331482 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature