Biochemical properties and subcellular localization of six members of the HXK family in maize and its metabolic contribution to embryo germination.

ABSTRACT:

Background

Seed germination is a crucial process in the plant life cycle when a dramatic variation of type and sugar content occurs just as the seed is hydrated. The production of hexose 6 phosphate is a key node in different pathways that are required for a successful germination. Hexokinase (HXK) is the only plant enzyme that phosphorylates glucose (Glc), so it is key to fueling several metabolic pathways depending on their substrate specificity, metabolite regulatory responses and subcellular localization. In maize, the HXK family is composed of nine genes, but only six of them (ZmHXK4-9) putatively encode catalytically active enzymes. Here, we cloned and functionally characterized putative catalytic enzymes to analyze their metabolic contribution during germination process.

SUBMITTER: Aguilera-Alvarado GP 

PROVIDER: S-EPMC6332545 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature