Sex influences DNA methylation and gene expression in human skeletal muscle myoblasts and myotubes.

ABSTRACT:

Background

Sex differences are known to impact muscle phenotypes, metabolism, and disease risk. Skeletal muscle stem cells (satellite cells) are important for muscle repair and to maintain functional skeletal muscle. Here we studied, for the first time, effects of sex on DNA methylation and gene expression in primary human myoblasts (activated satellite cells) before and after differentiation into myotubes.

Method

We used an array-based approach to analyse genome-wide DNA methylation and gene expression in myoblasts and myotubes from 13 women and 13 men. The results were followed up with a reporter gene assay.

Results

Genome-wide DNA methylation and gene expression differences between the sexes were detected in both myoblasts and myotubes, on the autosomes as well as

SUBMITTER: Davegardh C 

PROVIDER: S-EPMC6332625 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature