Role of colonic microbiota in the pathogenesis of ulcerative colitis.

ABSTRACT:

Background

Recent studies have found gut microbiota to be closely associated with onset and perpetuation of UC. Currently, studies about gut microbiota have mainly covered samples collected from the intestinal lumen. However, the luminal flora is only part of the gut microbiota. Studies of the changes in mucosal flora under pathological conditions have been lacking. In this study, we investigated the correlation between the onset of UC and flora changes in different intestinal layers.

Methods

The dextran sulfate sodium(DSS)-induced UC model was established by exposing mice to cycles of DSS. The luminal contents, an inner mucus layer, and outer mucus layer were harvested under sterile conditions. The samples were then analyzed using high-throughput sequencing of 16S rRNA V3 +

SUBMITTER: Pei LY 

PROVIDER: S-EPMC6332670 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature