Multimodal and Multiscale Analysis Reveals Distinct Vascular, Metabolic and Inflammatory Components of the Tissue Response to Limb Ischemia.

ABSTRACT: Ischemia triggers a complex tissue response involving vascular, metabolic and inflammatory changes.

Methods

We combined hybrid SPECT/CT or PET/CT nuclear imaging studies of perfusion, metabolism and inflammation with multicolor flow cytometry-based cell population analysis to comprehensively analyze the ischemic tissue response and to elucidate the cellular substrate of noninvasive molecular imaging techniques in a mouse model of hind limb ischemia.

Results

Comparative analysis of tissue perfusion with [^99mTc]-Sestamibi and arterial influx with [^99mTc]-labeled albumin microspheres by SPECT/CT revealed a distinct pattern of response to vascular occlusion: an early ischemic period of matched suppression of tissue perfusion and arterial influx, a subacute i

SUBMITTER: Kapanadze T 

PROVIDER: S-EPMC6332799 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature