An innovative "unlocked mechanism" by a double key avenue for one-pot detection of microRNA-21 and microRNA-141.

ABSTRACT: The accurate and quantitative detection of microRNAs (miRNAs) as next-generation, reliable biomarkers will provide vital information for cancer research and treatment. However, their unique, intrinsic features pose quite a challenge for miRNA profiling, especially for multiplexed detection. Thus, there is a strong and an ever-growing need to develop an accurate, simple, sensitive and specific miRNA sensing method. Methods: In this study, a simple and novel sensor is presented that uses a flow cytometry (FCM) method based on the double key "unlocked mechanism" and a fluorescence enrichment signal amplification strategy. The "unlocked mechanism" was cleverly designed via using hairpin DNA probes (HDs) labeled by fluorescent particles (FS) as the lock to block part of them, which can s

SUBMITTER: Peng W 

PROVIDER: S-EPMC6332803 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature