CDH1 somatic alterations in Mexican patients with diffuse and mixed sporadic gastric cancer.

ABSTRACT:

Background

Diffuse gastric cancer (DGC) is associated with the reduction or absence of the expression of the cell adhesion protein E-cadherin (encoded by the CDH1 gene). Molecular characteristics are less well described for mixed gastric cancer (MGC). The main somatic alterations that have been described in the CDH1 gene are mutations, loss of heterozygosity (LOH) and promoter methylation. The aim was to analyze CDH1 somatic alterations in Mexican patients with diffuse and mixed gastric cancer.

Methods

We searched for mutations in the CDH1 gene in tumor DNA from DGC (n = 13) and MGC (n = 7) patients by next generation sequencing (NGS). Validation of findings was performed using Sanger sequencing. LOH was analyzed using dinucleotide repeat markers surrounding the CDH1 gene, a

SUBMITTER: Bustos-Carpinteyro AR 

PROVIDER: S-EPMC6332846 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature