Genome-wide identification and characterization of transfer RNA-derived small RNAs in Plasmodium falciparum.

ABSTRACT:

Background

Transfer RNA (tRNA)-derived fragments (tRFs) have been widely identified in nature, functioning in diverse biological and pathological situations. Yet, the presence of these small RNAs in Plasmodium spp. remains unknown. Systematic identification and characterization of tRFs is therefore highly needed to understand further their roles in Plasmodium parasites, particularly in the virulent Plasmodium falciparum parasite.

Results

Genome-wide small RNAs with sizes ranging from 18-30 nucleotides from P. falciparum were deep-sequenced via Illumina HiSeq 2000 technology. In-depth analysis revealed the presence of a vast number of small RNAs originating from tRNA-coding genes, responsible for 22.4% of the total reads as the second predominant group. Three P. falciparum-de

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6332904 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature