Transfection of choanoflagellates illuminates their cell biology and the ancestry of animal septins.

ABSTRACT: As the closest living relatives of animals, choanoflagellates offer unique insights into animal origins and core mechanisms underlying animal cell biology. However, unlike traditional model organisms, such as yeast, flies, and worms, choanoflagellates have been refractory to DNA delivery methods for expressing foreign genes. Here we report a robust method for expressing transgenes in the choanoflagellate Salpingoeca rosetta, overcoming barriers that have previously hampered DNA delivery and expression. To demonstrate how this method accelerates the study of S. rosetta cell biology, we engineered a panel of fluorescent protein markers that illuminate key features of choanoflagellate cells. We then investigated the localization of choanoflagellate septins, a family of GTP-bindi

SUBMITTER: Booth DS 

PROVIDER: S-EPMC6333174 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature