The biosynthetic implications of late-stage condensation domain selectivity during glycopeptide antibiotic biosynthesis.

ABSTRACT: Non-ribosomal peptide synthesis is a highly important biosynthetic pathway for the formation of many secondary metabolites of medical relevance. Due to the challenges associated with the chemical synthesis of many of the products of these assembly lines, understanding the activity and selectivity of non-ribosomal peptide synthetase (NRPS) machineries is an essential step towards the redesign of such machineries to produce new bioactive peptides. Whilst the selectivity of the adenylation domains responsible for amino acid activation during NRPS synthesis has been widely studied, the selectivity of the essential peptide bond forming domains - known as condensation domains - is not well understood. Here, we present the results of a combination of in vitro and in vivo investigati

SUBMITTER: Schoppet M 

PROVIDER: S-EPMC6333238 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature