Palmitoylation controls trafficking of the intracellular Ca²⁺ channel MCOLN3/TRPML3 to regulate autophagy.

ABSTRACT: MCOLN3/TRPML3 is a Ca²⁺-permeable cation channel that is expressed in multiple subcellular compartments with dynamic localization. Our previous studies suggest that upon macroautophagy/autophagy induction MCOLN3/TRPML3 is recruited and provides Ca²⁺ for the fusion process in autophagosome biogenesis. However, how intracellular trafficking and the Ca²⁺ channel function of MCOLN3/TRPML3 are related to autophagy are not known. Here we report that MCOLN3/TRPML3 undergoes palmitoylation at its C-terminal region, which is required for dynamic trafficking and cellular function of MCOLN3/TRPML3 in autophagy. Palmitoylation regulated MCOLN3/TRPML3 surface expression and trafficking, but not channel properties or localization and function of intracellular MCOLN3/TRPM

SUBMITTER: Kim SW 

PROVIDER: S-EPMC6333453 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature