ATG5 is required for B cell polarization and presentation of particulate antigens.

ABSTRACT: The involvement of macroautophagy/autophagy proteins in B-cell receptor (BCR) trafficking, although suspected, is not well understood. We show that ATG5 (autophagy related 5) contributes to BCR polarization after stimulation and internalization into LAMP1 (lysosomal-associated membrane protein 1)⁺ and major histocompatibility complex class II (MHC-II)⁺ compartments. BCR polarization is crucial in the context of immobilized antigen processing. Moreover, antigen presentation to cognate T cells is decreased in the absence of ATG5 when the model antigen OVAL/ovalbumin is provided in an immobilized form in contrast to the normal presentation of soluble OVAL. We further show that ATG5 is required for centrosome polarization and actin nucleation in the immune synapse area. T

SUBMITTER: Arbogast F 

PROVIDER: S-EPMC6333460 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature