Modernization of Golgi staining techniques for high-resolution, 3-dimensional imaging of individual neurons.

ABSTRACT: Analysis of neuronal arborization and connections is a powerful tool in fundamental and clinical neuroscience. Changes in neuronal morphology are central to brain development and plasticity and are associated with numerous diseases. Golgi staining is a classical technique based on a deposition of metal precipitate in a random set of neurons. Despite their versatility, Golgi methods have limitations that largely precluded their use in advanced microscopy. We combined Golgi staining with fluorescent labeling and tissue clearing techniques in an Alzheimer's disease model. We further applied 3D electron microscopy to visualize entire Golgi-stained neurons, while preserving ultrastructural details of stained cells, optimized Golgi staining for use with block-face scanning electron microscopy, a

SUBMITTER: Vints K 

PROVIDER: S-EPMC6333844 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature