The inflammasome adapter ASC assembles into filaments with integral participation of its two Death Domains, PYD and CARD.

ABSTRACT: The inflammasome is a multiprotein complex necessary for the onset of inflammation. The adapter protein ASC assembles inflammasome components by acting as a molecular glue between danger-signal sensors and procaspase-1. The assembly is mediated by ASC self-association and protein interactions via its two Death Domains, PYD and CARD. Truncated versions of ASC have been shown to form filaments, but information on the filaments formed by full-length ASC is needed to construct a meaningful model of inflammasome assembly. To gain insights into this system, we used a combination of transmission EM, NMR, and computational analysis to investigate intact ASC structures. We show that ASC forms ∼6-7-nm-wide filaments that stack laterally to form bundles. The structural characteristics and dimensions

SUBMITTER: Nambayan RJT 

PROVIDER: S-EPMC6333874 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature