Gβγ signaling to the chemotactic effector P-REX1 and mammalian cell migration is directly regulated by Gα_q and Gα₁₃ proteins.

ABSTRACT: G protein-coupled receptors stimulate Rho guanine nucleotide exchange factors that promote mammalian cell migration. Rac and Rho GTPases exert opposing effects on cell morphology and are stimulated downstream of Gβγ and Gα_12/13 or Gα_q, respectively. These Gα subunits might in turn favor Rho pathways by preventing Gβγ signaling to Rac. Here, we investigated whether Gβγ signaling to phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchange factor 1 (P-REX1), a key Gβγ chemotactic effector, is directly controlled by Rho-activating Gα subunits. We show that pharmacological inhibition of Gα_q makes P-REX1 activation by G_q/G_i-coupled lysophosphatidic acid receptors more effective. Moreover, chemogenetic control of G_i and G

SUBMITTER: Cervantes-Villagrana RD 

PROVIDER: S-EPMC6333895 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature