Orthogonal Comparison of GC-MS and ¹H NMR Spectroscopy for Short Chain Fatty Acid Quantitation.

ABSTRACT: Short chain fatty acids (SCFAs) are important regulators of host physiology and metabolism and may contribute to obesity and associated metabolic diseases. Interest in SCFAs has increased in part due to the recognized importance of how production of SCFAs by the microbiota may signal to the host. Therefore, reliable, reproducible, and affordable methods for SCFA profiling are required for accurate identification and quantitation. In the current study, four different methods for SCFA (acetic acid, propionic acid, and butyric acid) extraction and quantitation were compared using two independent platforms including gas chromatography coupled with mass spectrometry (GC-MS) and ¹H nuclear magnetic resonance (NMR) spectroscopy. Sensitivity, recovery, repeatability, matrix effect, and

SUBMITTER: Cai J 

PROVIDER: S-EPMC6334302 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature