Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging.

ABSTRACT:

Background

HIV infection is enhanced by cell adhesions that form between infected and uninfected T cells called virological synapses (VS). VS are initiated by an interaction between Env and CD4 on cell surfaces and result in the recruitment of virus assembly to the site of cell-cell contact. However, the recruitment of Env to the VS and its relationship to Gag recruitment is not well defined.

Results

To study the trafficking of HIV-1 Env through the VS, we constructed a molecular clone of HIV carrying a green fluorescent protein-Env fusion protein called, HIV Env-isfGFP-∆V1V2. The Env-isfGFP-∆V1V2 fusion protein does not produce virus particles on its own, but can be rescued by cotransfection with full-length HIV constructs and produce virus particles that package the fluore

SUBMITTER: Wang L 

PROVIDER: S-EPMC6334456 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature