Overexpression of CCDC69 activates p14^ARF/MDM2/p53 pathway and confers cisplatin sensitivity.

ABSTRACT:

Objectives

The aim of the study is to explore the relationship between CCDC69 expression and resistance of ovarian cancer cells to cisplatin and reveal the underlying mechanism.

Methods

One hundred thirty five ovarian cancer patients with intact chemo-response information from The Cancer Genome Atlas (TCGA) database were included and analyzed. Stable CCDC69 overexpressing 293 and ovarian cancer A2780 cell lines were established and subjected to examine cell apoptosis and cell cycle distribution using CCK-8 assay and flow cytometry. Cell cycle and apoptosis pathway were evaluated by immunoblots. Stability of p14^ARF/MDM2/p53 pathway related proteins were determined by half-life analysis and ubiquitination experiments.

Results

We found that CCDC69 expression w

SUBMITTER: Cui L 

PROVIDER: S-EPMC6334460 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature