Distinct roles of Dlk1 isoforms in bi-potential differentiation of hepatic stem cells.

ABSTRACT:

Background

Fully understanding the developmental process of hepatic stem cells (HSCs) and the mechanisms of their committed differentiation is essential for optimizing the generation of functional hepatocytes for cell therapy in liver disease. Delta-like 1 homolog (Dlk1), primarily the membrane-bound form (Dlk1^M), is generally used as a surface marker for fetal hepatic stem cell isolation, while its soluble form (Dlk1^S) and the functional roles of different Dlk1 isoforms in HSC differentiation remain to be investigated.

Methods

Hepatic spheroid-derived cells (HSDCs) were isolated from E12.5 mouse livers to obtain Dlk1⁺ and Dlk1^-subpopulations. Colony formation, BrdU staining, and CCK8 assays were used to evaluate the cell prolifera

SUBMITTER: Huang J 

PROVIDER: S-EPMC6334473 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature