A lysosomal chloride ion-selective fluorescent probe for biological applications.

ABSTRACT: Lysosomal pHs are maintained at low values by the cooperative action of a proton pump and a chloride channel to maintain electroneutrality. Owing to the biological significance of lysosomal chloride ions, measurements of their levels are of great importance to understand lysosome-associated biological events. However, appropriate probes to selectively detect Cl^- ions within acidic lysosomes have not been developed to date. In this study, we prepared MQAE-MP, a lysosomal Cl^--selective fluorescent probe, and applied it to gain information about biological processes associated with lysosomes. The fluorescence of MQAE-MP is pH-insensitive over physiological pH ranges and is quenched by Cl^- with a Stern-Volmer constant of 204 M^-1. Because MQAE-MP dete

SUBMITTER: Park SH 

PROVIDER: S-EPMC6334773 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature