Therapeutic Fc fusion protein misfolding: A three-phasic cultivation experimental design.

ABSTRACT: Cell culture process optimization is a critical solution to most of the challenges faced by the pharmaceutical manufacturing. One of the major problems encountered in large-scale production of therapeutic proteins is misfolded protein production. The accumulation of misfolded therapeutic proteins is an immunogenic signal and a risk factor for immunogenicity of the final product. The aim of this study was the statistical optimization of three-phasic temperature shift and timing for enhanced production of correctly folded Fc-fusion protein. The effect of culture temperatures were investigated using the biphasic culture system. Box-Behnken design was then used to compute temperature and time of shifting optimum. Response surface methodology revealed that maximum production with low level of m

SUBMITTER: Ghorbani Aghdam A 

PROVIDER: S-EPMC6334962 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature