Phagocytosis of microparticles by alveolar macrophages during acute lung injury requires MerTK.

ABSTRACT: Microparticles are a newly recognized class of mediators in the pathophysiology of lung inflammation and injury, but little is known about the factors that regulate their accumulation and clearance. The primary objective of our study was to determine whether alveolar macrophages engulf microparticles and to elucidate the mechanisms by which this occurs. Alveolar microparticles were quantified in bronchoalveolar fluid of mice with lung injury induced by LPS and hydrochloric acid. Microparticle numbers were greatest at the peak of inflammation and declined as inflammation resolved. Isolated, fluorescently labeled particles were placed in culture with macrophages to evaluate ingestion in the presence of endocytosis inhibitors. Ingestion was blocked with cytochalasin D and wortmannin, consiste

SUBMITTER: Mohning MP 

PROVIDER: S-EPMC6335009 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature