11,12 and 14,15 epoxyeicosatrienoic acid rescue deteriorated wound healing in ischemia.

ABSTRACT:

Introduction

Epoxyeicosatrienoic acids (EETs) are able to enhance angiogenesis and regulate inflammation that is especially important in wound healing under ischemic conditions. Thus, we evaluated the effect of local EET application on ischemic wounds in mice.

Methods

Ischemia was induced by cautherization of two of the three supplying vessels to the mouse ear. Wounding was performed on the ear three days later. Wounds were treated either with 11,12 or 14,15 EET and compared to untreated control and normal wounds. Epithelialization was measured every second day. VEGF, TNF-α, TGF-β, matrix metalloproteinases (MMP), tissue inhibitors of metalloproteinases (TIMP), Ki67, and SDF-1α were evaluated immunohistochemically in wounds on day 3, 6, and 9.

Results

Ischemia delayed

SUBMITTER: Sommer K 

PROVIDER: S-EPMC6335021 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature