In vitro model reveals a role for mechanical stretch in the remodeling response of lymphatic muscle cells.

ABSTRACT:

Objective

Using primary LMCs in vitro, we sought to characterize the impact of LMC remodeling on their functional and molecular response to mechanical loading and culture conditions.

Methods

Primary "wounded leg" LMCs were derived from the hindlimb of three sheep who underwent lymphatic injury 6 weeks prior, while "control leg" LMCs were derived from the contralateral, unwounded, limb. Function of the LMCs was characterized in response to media of variable levels of serum (10% vs 0.2%) and glucose (4.5 vs 1 g/L). Functional and proteomic data were evaluated in LMCs exposed to cyclic stretch (0.1 Hz, 7.5% elongation) for 1 week.

Results

LMCs were sensitive to changes in serum levels, significantly reducing overall activity and collagen synthesis under low serum conditi

SUBMITTER: Hooks JST 

PROVIDER: S-EPMC6335159 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature