Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression.

ABSTRACT:

Background

Currently no methods are available to predict the clinical outcome of individual horses with equine sarcoid (ES) disease.

Objective

To investigate if whole blood microRNA (miRNA) profiles can predict the long-term development of ES tumors.

Animals

Five horses with regression and 5 with progression of ES lesions monitored over 5-7 years and 5 control horses free of ES for at least 5 years.

Methods

For this cohort study, RNA extracted from whole blood samples from the regression, progression, and control groups was used for high throughput sequencing. Known and novel miRNAs were identified using miRDeep2 and differential expression analysis was carried out by the DESeq2 algorithm. Target gene and pathway prediction as well as enrichment and network ana

SUBMITTER: Unger L 

PROVIDER: S-EPMC6335546 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature