Estrogen receptor signaling regulates the expression of the breast tumor kinase in breast cancer cells.

ABSTRACT:

Background

BRK is, a non-receptor tyrosine kinase, overexpressed in approximately 85% of human invasive ductal breast tumors. It is not clear whether BRK expression correlates with breast cancer subtypes, or the expression has prognostic or diagnostic significance. Herein, we investigated the correlation of BRK with any breast cancer subtypes and clinicopathological significance of BRK expression in breast cancer.

Methods

In this study, we examined BRK expression in 120 breast tumor samples and 29 breast cancer cell lines to explore the positive correlation between BRK and the expression of ERα. We used immunohistochemistry, RT-PCR, and immunoblotting to analyse our experimental samples.

Result

We demonstrate that estrogen induces BRK gene and protein expression in ER

SUBMITTER: Miah S 

PROVIDER: S-EPMC6335685 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature