Combinatorial approach for improved cyanidin 3-O-glucoside production in Escherichia coli.

ABSTRACT:

Background

Multi-monocistronic and multi-variate vectors were designed, built, and tested for the improved production of cyanidin 3-O-glucoside (C3G) in Escherichia coli BL21 (DE3). The synthetic bio-parts were designed in such a way that multiple genes can be assembled using the bio-brick system, and expressed under different promoters in a single vector. The vectors harbor compatible cloning sites, so that the genes can be shuffled from one vector to another in a single step, and assembled into a single vector. The two required genes: anthocyanidin synthase (PhANS) from Petunia hybrida, and cyanidin 3-O-glucosyltransferase (At3GT) from Arabidopsis thaliana, were individually cloned under P_T7, P_trc, and P_lacUV5 promoters. Both PhANS and At3GT were

SUBMITTER: Shrestha B 

PROVIDER: S-EPMC6335687 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature