Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis.

ABSTRACT:

Background

Ell3 is a RNA polymerase II elongation factor that has various cell type-dependent functions, such as regulating the differentiation efficiency of embryonic stem cells and sensitizing cancer cells to anticancer drugs. However, there has been little research on the role of Ell3 on the regulation of senescence and apoptosis of stem cells.

Methods

We analyzed the senescence of Ell3-suppressed stem cells by mitochondrial activity, β-gal (+) cells, and lineage differentiation efficiency. The apoptosis of Ell3-overexpressing stem cells was analyzed by Annexin V staining, Immunoblot, and Live&dead assay. In addition, chromatin immunoprecipitation and luciferase assays were used to demonstrate p53 functions as a direct transcriptional activator of Ell3.

Results

Sup

SUBMITTER: Lee JY 

PROVIDER: S-EPMC6335702 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature