Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.

ABSTRACT: All biosensing platforms rest on two pillars: specific biochemical recognition of a particular analyte and transduction of that recognition into a readily detectable signal. Most existing biosensing technologies utilize proteins that passively bind to their analytes and therefore require wasteful washing steps, specialized reagents, and expensive instruments for detection. To overcome these limitations, protein engineering strategies have been applied to develop new classes of protein-based sensor/actuators, known as protein switches, responding to small molecules. Protein switches change their active state (output) in response to a binding event or physical signal (input) and therefore show a tremendous potential to work as a biosensor. Synthetic protein switches can be created by the fus

SUBMITTER: Ribeiro LF 

PROVIDER: S-EPMC6335823 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature