Thermodynamic Insights into the Separation of Carotenoids in Reversed-Phase Liquid Chromatography.

ABSTRACT: The retention mechanism of four major carotenoids, two xanthophylls (i.e., lutein and zeaxanthin) and two carotenes (i.e., lycopene and β-carotene), was investigated in reversed-phase liquid chromatography with the aim of thermodynamic analysis. The experimental variables considered in this study were the composition of mobile phase (MP) and the temperature. Chromatographic elutions were undertaken under linear, isocratic conditions by using a C18 stationary phase, four different MP compositions (by varying the ratio methanol/acetonitrile from 66.5/28.5 to 47.5/47.5 v/v), and column temperatures in the range 283-313 K. Traditional Van't Hoff analysis has been used to estimate changes of standard enthalpy (ΔH°) and Gibbs free energy (ΔG°) associated with the solute tran

SUBMITTER: Marchetti N 

PROVIDER: S-EPMC6335859 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature