Investigation of Gene Expressions of Myeloma Cells in the Bone Marrow of Multiple Myeloma Patients by Transcriptome Analysis

ABSTRACT:

Background

Multiple myeloma is a plasma cell dyscrasia characterized by transformation of B cells into malignant cells. Although there are data regarding the molecular pathology of multiple myeloma, the molecular mechanisms of the disease have not been fully elucidated.

Aims

To investigate the gene expression profiles in bone marrow myeloma cells via RNA-sequencing technology.

Study design

Cell study.

Methods

Myeloma cells from four patients with untreated multiple myeloma and B cells from the bone marrow of four healthy donors were sorted using a FACSAria II flow cytometer. The patient pool of myeloma cells and the control pool of B cells were the two comparative groups. A transcriptome analysis was performed and the results were analyzed using bioinformatics

SUBMITTER: Sarıman M 

PROVIDER: S-EPMC6335938 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature