Checkpoint Blockade Immunotherapy Induces Dynamic Changes in PD-1^-CD8⁺ Tumor-Infiltrating T Cells.

ABSTRACT: An improved understanding of the anti-tumor CD8⁺ T cell response after checkpoint blockade would enable more informed and effective therapeutic strategies. Here we examined the dynamics of the effector response of CD8⁺ tumor-infiltrating lymphocytes (TILs) after checkpoint blockade therapy. Bulk and single-cell RNA profiles of CD8⁺ TILs after combined Tim-3+PD-1 blockade in preclinical models revealed significant changes in the transcriptional profile of PD-1^- TILs. These cells could be divided into subsets bearing characterstics of naive-, effector-, and memory-precursor-like cells. Effector- and memory-precursor-like TILs contained tumor-antigen-specific cells, exhibited proliferative and effector capacity, and expanded in response to different

SUBMITTER: Kurtulus S 

PROVIDER: S-EPMC6336113 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature