Atrial-Specific Gene Delivery Using an Adeno-Associated Viral Vector.

ABSTRACT:

Rationale

Somatic overexpression in mice using an adeno-associated virus (AAV) as gene transfer vectors has become a valuable tool to analyze the roles of specific genes in cardiac diseases. The lack of atrial-specific AAV vector has been a major obstacle for studies into the pathogenesis of atrial diseases. Moreover, gene therapy studies for atrial fibrillation would benefit from atrial-specific vectors. Atrial natriuretic factor (ANF) promoter drives gene expression specifically in atrial cardiomyocytes.

Objective

To establish the platform of atrial specific in vivo gene delivery by AAV-ANF.

Methods and results

We constructed AAV vectors based on serotype 9 (AAV9) that are driven by the atrial-specific ANF promoter. Hearts from mice injected with AAV9-ANF-GFP (green

SUBMITTER: Ni L 

PROVIDER: S-EPMC6336523 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature