Characterization of the Src-regulated kinome identifies SGK1 as a key mediator of Src-induced transformation.

ABSTRACT: Despite significant progress, our understanding of how specific oncogenes transform cells is still limited and likely underestimates the complexity of downstream signalling events. To address this gap, we use mass spectrometry-based chemical proteomics to characterize the global impact of an oncogene on the expressed kinome, and then functionally annotate the regulated kinases. As an example, we identify 63 protein kinases exhibiting altered expression and/or phosphorylation in Src-transformed mammary epithelial cells. An integrated siRNA screen identifies nine kinases, including SGK1, as being essential for Src-induced transformation. Accordingly, we find that Src positively regulates SGK1 expression in triple negative breast cancer cells, which exhibit a prominent signalling network gove

SUBMITTER: Ma X 

PROVIDER: S-EPMC6336867 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature