Ontology highlight
ABSTRACT:
SUBMITTER: Quehenberger J
PROVIDER: S-EPMC6337131 | biostudies-literature | 2019 Jan
REPOSITORIES: biostudies-literature
Quehenberger Julian J Reichenbach Tom T Baumann Niklas N Rettenbacher Lukas L Divne Christina C Spadiut Oliver O
International journal of molecular sciences 20190106 1
While in search of an enzyme for the conversion of xylose to xylitol at elevated temperatures, a xylose reductase (XR) gene was identified in the genome of the thermophilic fungus <i>Chaetomium thermophilum</i>. The gene was heterologously expressed in <i>Escherichia coli</i> as a His6-tagged fusion protein and characterized for function and structure. The enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. A homology mo ...[more]