Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.

ABSTRACT:

Background

Many valuable biopharmaceutical and biotechnological proteins have been produced in Escherichia coli, however these proteins are almost exclusively localised in the cytoplasm or periplasm. This presents challenges for purification, i.e. the removal of contaminating cellular constituents. One solution is secretion directly into the surrounding media, which we achieved via the 'hijack' of the flagellar type III secretion system (FT3SS). Ordinarily flagellar subunits are exported through the centre of the growing flagellum, before assembly at the tip. However, we exploit the fact that in the absence of certain flagellar components (e.g. cap proteins), monomeric flagellar proteins are secreted into the supernatant.

Results

We report the creation and iterative improvem

SUBMITTER: Green CA 

PROVIDER: S-EPMC6337784 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature