Liver DNA methylation of FADS2 associates with FADS2 genotype.

ABSTRACT:

Background

Non-alcoholic fatty liver disease has been associated with increased mRNA expression of FADS2 in the liver and estimated activity of delta-6 desaturase in serum, encoded by the FADS2 gene. Since DNA methylation in the FADS1/2/3 gene cluster has been previously linked with genetic variants and desaturase activities, we now aimed to discover factors regulating DNA methylation of the CpG sites annotated to FADS1/2 genes.

Methods

DNA methylation levels in the CpG sites annotated to FADS2 and FADS1 were analyzed from liver samples of 95 obese participants of the Kuopio Obesity Surgery Study (34 men and 61 women, age 49.5 ± 7.7 years, BMI 43.0 ± 5.7 kg/m²) using the Infinium HumanMethylation450 BeadChip (Illumina). Associations between DNA methylation levels

SUBMITTER: Walle P 

PROVIDER: S-EPMC6337806 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature