Nuclear envelope expansion in budding yeast is independent of cell growth and does not determine nuclear volume.

ABSTRACT: Most cells exhibit a constant ratio between nuclear and cell volume. The mechanism dictating this constant ratio and the nuclear component(s) that scale with cell size are not known. To address this, we examined the consequences to the size and shape of the budding yeast nucleus when cell expansion is inhibited by down-regulating components of the secretory pathway. We find that under conditions where cell size increase is restrained, the nucleus becomes bilobed, with the bulk of the DNA in one lobe and the nucleolus in the other. The formation of bilobed nuclei is dependent on fatty acid and phospholipid synthesis, suggesting that it is associated with nuclear membrane expansion. Bilobed nuclei appeared predominantly after spindle pole body separation, suggesting that nuclear envelope exp

SUBMITTER: Walters AD 

PROVIDER: S-EPMC6337908 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature