Impact of broad regulatory regions on Gdf5 expression and function in knee development and susceptibility to osteoarthritis.

ABSTRACT:

Objectives

Given the role of growth and differentiation factor 5 (GDF5) in knee development and osteoarthritis risk, we sought to characterise knee defects resulting from Gdf5 loss of function and how its regulatory regions control knee formation and morphology.

Methods

The brachypodism (bp) mouse line, which harbours an inactivating mutation in Gdf5, was used to survey how Gdf5 loss of function impacts knee morphology, while two transgenic Gdf5 reporter bacterial artificial chromosome mouse lines were used to assess the spatiotemporal activity and function of Gdf5 regulatory sequences in the context of clinically relevant knee anatomical features.

Results

Knees from homozygous bp mice (bp/bp) exhib

SUBMITTER: Pregizer SK 

PROVIDER: S-EPMC6338229 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature