A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria.

ABSTRACT: The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle-specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity (η_m ) and micropolarity (ϵ_m ), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of η_m and ϵ_m by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of η_m and ϵ_m in the presence of

SUBMITTER: Jimenez-Sanchez A 

PROVIDER: S-EPMC6338234 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature