Chromosomal scan of single sperm cells by combining fluorescence-activated cell sorting and next-generation sequencing.

ABSTRACT:

Purpose

The purpose of this study was to develop a feasible approach for single sperm isolation and chromosome analysis by next-generation sequencing (NGS).

Methods

Single sperm cells were isolated from semen samples of normozoospermic male and an infertile reciprocal translocation (RcT) carrier with the 46,XY,t(7;13)(p12;q12.1) karyotype using the optimized fluorescence-activated cell sorting (FACS) technique. Genome profiling was performed using NGS.

Results

Following whole-genome amplification, NGS, and quality control, the final chromosome analysis was performed on 31 and 6 single cell samples derived from the RcT carrier and normozoospermic male, respectively. All sperm cells from normozoospermic male showed a normal haploid 23-chromosome profile. For the RcT car

SUBMITTER: Tran QT 

PROVIDER: S-EPMC6338602 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature