Kinetics of corneal leukocytes by intravital multiphoton microscopy.

ABSTRACT: Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing the means to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation. Significant alterations in size and morphology of corneal CD11c⁺ dendritic cells (DCs) were noted following acute sterile inflammation, including cell volume (4364.4 ± 489.6 vs. 1787.6 ± 111.0 μm³, P < 0.001) and sphericity (0.8

SUBMITTER: Seyed-Razavi Y 

PROVIDER: S-EPMC6338630 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature