Urinary concentrating defect in mice lacking Epac1 or Epac2.

ABSTRACT: cAMP is a universal second messenger regulating a plethora of processes in the kidney. Two downstream effectors of cAMP are PKA and exchange protein directly activated by cAMP (Epac), which, unlike PKA, is often linked to elevation of [Ca²⁺]_i. While both Epac isoforms (Epac1 and Epac2) are expressed along the nephron, their relevance in the kidney remains obscure. We combined ratiometric calcium imaging with quantitative immunoblotting, immunofluorescent confocal microscopy, and balance studies in mice lacking Epac1 or Epac2 to determine the role of Epac in renal water-solute handling. Epac1^-/- and Epac2^-/- mice developed polyuria despite elevated arginine vasopressin levels. We did not detect major deficiencies in arginine vasopressin [Ca²⁺

SUBMITTER: Cherezova A 

PROVIDER: S-EPMC6338637 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature