Endothelin receptor A and p66Shc regulate spontaneous Ca²⁺ oscillations in smooth muscle cells controlling renal arterial spontaneous motion.

ABSTRACT: Adaptor protein p66Shc is overexpressed in smooth muscle cells of renal resistance vessels of hypertensive salt-sensitive rats and is involved in the regulation of renal vascular tone. We applied 2-photon laser scanning fluorescence microscopy to analyze spontaneous dynamic fluctuations in intracellular calcium concentrations ([Ca²⁺]_i) in smooth muscle cells embedded in the walls of freshly isolated renal resistance arteries. The amplitude, number of events, and frequency of spontaneous [Ca²⁺]_i oscillations triggered by endogenously released endothelin-1 were recorded in smooth muscle cells of the renal arteries. Endothelin receptor A antagonist BQ123 dramatically reduced the amplitude and frequency of spontaneous Ca²⁺ events, producin

SUBMITTER: Palygin O 

PROVIDER: S-EPMC6338658 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature