Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.

ABSTRACT: Respiratory viral infections often mimic the symptoms of infections caused by bacteria; however, restricted and targeted administration of antibiotics is needed to combat growing antimicrobial resistance. This is particularly relevant in low-income settings. In this work, we describe the use of isothermal amplification of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles. Two oligonucleotide probes, one in-house designed and one known adenoviral probe were tested and validated for microarray detection down to 50 nM using a synthetic target template. Furthermore, primers were shown to function in a recombinase polymerase amplification reaction using both synthetic templ

SUBMITTER: Nybond S 

PROVIDER: S-EPMC6338793 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature