Obesity impacts the regulation of miR-10b and its targets in primary breast tumors.

ABSTRACT:

Background

Obesity increases breast cancer (BC) risk in post-menopausal women by mostly unknown molecular mechanisms which may partly be regulated by microRNAs (miRNAs).

Methods

We isolated RNA from paired benign and malignant biopsies from 83 BC patients and determined miRNA profiles in samples from 12 women at the extremes of the BMI distribution by RNA-seq. Candidates were validated in all samples. Associations between miR-10b expression and validated target transcript levels, and effects of targeted manipulation of miR-10b levels in a primary BC cell line on proliferation and invasion potential, were explored.

Results

Of the 148 miRNAs robustly expressed in breast tissues, the levels of miR-21, miR-10b, miR-451a, miR-30c, and miR-378d were significantly associated

SUBMITTER: Meerson A 

PROVIDER: S-EPMC6339293 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature