Comparative analysis of differential gene expression analysis tools for single-cell RNA sequencing data.

ABSTRACT:

Background

The analysis of single-cell RNA sequencing (scRNAseq) data plays an important role in understanding the intrinsic and extrinsic cellular processes in biological and biomedical research. One significant effort in this area is the detection of differentially expressed (DE) genes. scRNAseq data, however, are highly heterogeneous and have a large number of zero counts, which introduces challenges in detecting DE genes. Addressing these challenges requires employing new approaches beyond the conventional ones, which are based on a nonzero difference in average expression. Several methods have been developed for differential gene expression analysis of scRNAseq data. To provide guidance on choosing an appropriate tool or developing a new one, it is necessary to evaluate and co

SUBMITTER: Wang T 

PROVIDER: S-EPMC6339299 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature