Utilising datasheets for the informed automated design and build of a synthetic metabolic pathway.

ABSTRACT:

Background

The automation of modular cloning methodologies permits the assembly of many genetic designs. Utilising characterised biological parts aids in the design and redesign of genetic pathways. The characterisation information held on datasheets can be used to determine whether a biological part meets the design requirements. To manage the design of genetic pathways, researchers have turned to modelling-based computer aided design software tools.

Result

An automated workflow has been developed for the design and build of heterologous metabolic pathways. In addition, to demonstrate the powers of electronic datasheets we have developed software which can transfer part information from a datasheet to the Design of Experiment software JMP. To this end we were able to use De

SUBMITTER: Exley K 

PROVIDER: S-EPMC6339355 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature