Reconstruction of full-length circular RNAs enables isoform-level quantification.

ABSTRACT: Currently, circRNA studies are shifting from the identification of circular transcripts to understanding their biological functions. However, such endeavors have been limited by large-scale determination of their full-length sequences and also by the inability of accurate quantification at the isoform level. Here, we propose a new feature, reverse overlap (RO), for circRNA detection, which outperforms back-splice junction (BSJ)-based methods in identifying low-abundance circRNAs. By combining RO and BSJ features, we present a novel approach for effective reconstruction of full-length circRNAs and isoform-level quantification from the transcriptome. We systematically compared the difference between the BSJ-level and isoform-level differential expression analyses using human liver tumor and

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC6339429 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature