Optical Clearing in the Kidney Reveals Potassium-Mediated Tubule Remodeling.

ABSTRACT: Distal nephron remodeling contributes to the pathophysiology of many clinically relevant scenarios, including diuretic resistance and certain Mendelian disorders of blood pressure. However, constitutive genetic disruptions are likely to have substantial developmental effects in this segment, and whether tubule remodeling upon physiological stimuli is a normal homeostatic mechanism is not known. Since the distal nephron acts as a potassium sensor, we assessed proliferation and tubule length in three dimensions upon dietary or inducible genetic manipulation by using optical clearing of adult mouse kidneys, whole-mount immunolabeling, and advanced light microscopy. We show that dietary potassium restriction leads promptly to proliferation of various nephron segments, including the distal conv

SUBMITTER: Saritas T 

PROVIDER: S-EPMC6339512 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature